What evolutionary event characterized the ancestral lineage of rattlesnakes before the major diversification into modern, venomous New World pit vipers?

Answer

Deletion of several key venom-related genes, resulting in a period of venom loss

The evolutionary history leading to modern rattlesnakes involved a complex process that included significant reversals. Early in their lineage, the ancestors of modern rattlesnakes underwent a genetic 'reset' or reduction where they actually deleted several genes crucial for venom production. This meant that for a period, these ancestors were not venomous in the way related species were. This loss phase occurred before the major diversification event that ultimately resulted in the highly venomous pit vipers seen today, which subsequently reacquired or repurposed the necessary genes through duplication events to form their complex venom systems.

What evolutionary event characterized the ancestral lineage of rattlesnakes before the major diversification into modern, venomous New World pit vipers?
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