What technique was instrumental in redrawing the lines of descent within the Pantherophis genus?
Answer
Systematic investigation using molecular data, like DNA sequences
The unraveling of the *P. obsoletus* complex and the redrawing of evolutionary relationships were significantly advanced by systematic biological investigation employing molecular data. Specifically, research involving the analysis of DNA sequences has provided a much clearer picture of evolutionary distance and relationships within the *Pantherophis* genus than external morphological features could alone reveal. These genetic markers highlight deep separations between populations, helping to establish which groups truly represent distinct evolutionary units and indicating ancient divergence times.

Related Questions
What primary diagnostic tool led to the initial grouping under Pantherophis obsoletus?What technique was instrumental in redrawing the lines of descent within the Pantherophis genus?Which specific rat snake population was once nested within P. obsoletus but is now recognized as Pantherophis spiloides?What term is used to describe the closely related species formerly grouped erroneously under P. obsoletus?What key distinguishing trait is typically associated with adult Black Rat Snakes (P. alleghaniensis)?Which geographical feature is suggested as a potential ancient barrier that reinforced genetic separation between eastern and western groups?What is a physical feature sometimes noted in Western Rat Snakes compared to smoother eastern relatives?What is the current generalized status assigned to the Gray Rat Snake component?What conservation implication arises from splitting the widespread P. obsoletus into multiple species?What did the original, broad application of Pantherophis obsoletus historically encompass?