Approximately what percentage of the Wood Frog's body water can form ice crystals during its frozen state?

Answer

Up to 65%.

The wood frog possesses an extraordinary capability among vertebrates: surviving being completely frozen solid during harsh winter periods. This process involves the external formation of ice crystals within the frog's body tissues. It is documented that ice can form and fill up to approximately 65% of the frog's total body water content. The critical aspect of this physiological feat is that the ice formation must occur *outside* of the cells themselves. This is managed by the intense production of glucose within the vital organs, which acts as a natural cryoprotectant, shielding the cellular structures from fatal dehydration and physical damage caused by the expanding ice matrix surrounding them.

Approximately what percentage of the Wood Frog's body water can form ice crystals during its frozen state?

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