What is the primary physical concern for the anole during handling?

Tail autotomy

Although green anoles are capable of biting if they feel severely threatened or mishandled, the most significant physical risk posed to the lizard during improper capture or rough handling is tail autotomy, which is the deliberate shedding of the tail. While anoles can often survive the loss of their tail, the act itself represents a massive expenditure of stored energy, which is critical for their survival and well-being. This defense mechanism, though a survival tool, leaves the animal vulnerable and drained, making its prevention the chief priority when any necessary physical interaction, such as cage relocation, is required.